386 CRISPR-Cas9 nucleases are widely used for genome editing, but the range of sequences that Cas9 can recognize is constrained by the need for a specific protospacer adjacent motif (PAM); here the commonly used Streptococcus pyogenes Cas9 (SpCas9) is modified to recognize alternative PAM sequences, enabling robust editing of endogenous gene sites in zebrafish and human cells not currently targetable by wild-type SpCas9.
http://www.nature.com/nature/journal/v523/n7561/full/nature14592.html
385 Tyrosine glycosylation of Rho by Yersinia toxin impairs blastomere cell behaviour in zebrafish embryos.
http://www.nature.com/ncomms/2015/150720/ncomms8807/full/ncomms8807.html
384 Cyclic di-GMP acts as a cell cycle oscillator to drive chromosome replication.
http://www.nature.com/nature/journal/v523/n7559/full/nature14473.html
383 A simple method for generating high-resolution maps of genome-wide protein binding.
http://elifesciences.org/content/4/e09225
382 Aminoacylating Urzymes Challenge the RNA World Hypothesis.
http://www.jbc.org/content/288/37/26856
381 Defining fundamental steps in the assembly of the Drosophila RNAi enzyme complex.
http://www.nature.com/nature/journal/v521/n7553/full/nature14254.html
380 CRISPR gene editing.
http://www.pnas.org/content/112/20/6245.extract
379 The mechanisms by which Xist, a long non-coding RNA, silences one X chromosome in female mammals are unknown; here a mass spectrometry-based approach is developed to identify several proteins that interact directly with Xist, including the transcriptional repressor SHARP that is required for transcriptional silencing through the histone deacetylase HDAC3.
http://www.nature.com/nature/journal/v521/n7551/full/nature14443.html
378 Phosphorylation of GATA-6 is required for vascular smooth muscle cell differentiation after mTORC1 inhibition.
http://stke.sciencemag.org/content/8/376/ra44.abstract
377 Phased synthesis of germline-protective piRNAs along precursor RNAs increases piRNA sequence diversity.
http://www.sciencemag.org/content/348/6236/812.abstract
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