Biotechnology

Articles:

456  An Atmosphere‐Breathing Refillable Biphasic Device for Cell Replacement Therapy.
https://onlinelibrary.wiley.com/doi/10.1002/adma.201905135

455  Harnessing type I CRISPR–Cas systems for genome engineering in human cells.
https://www.nature.com/articles/s41587-019-0310-0

454  Visualizing structure and transitions in high-dimensional biological data.
https://www.nature.com/articles/s41587-019-0336-3

453  Carboxylated branched poly(β-amino ester) nanoparticles enable robust cytosolic protein delivery and CRISPR-Cas9 gene editing.
https://advances.sciencemag.org/content/5/12/eaay3255

452  Proof of concept for identifying cystic fibrosis from perspiration samples.
https://www.pnas.org/content/116/49/24408

451  A high-throughput method for amplication-free single-cell whole-genome sequencing can be scaled up to analyze tens of thousands of cells from different tissues and clinical sample types and identifies replication states, aneuploidies, and subclonal mutations.
https://www.cell.com/cell/fulltext/S0092-8674(19)31176-6

450  Targeted homology-directed repair in blood stem and progenitor cells with CRISPR nanoformulations.
https://www.nature.com/articles/s41563-019-0385-5

449  Search-and-replace genome editing without double-strand breaks or donor DNA.
https://www.nature.com/articles/s41586-019-1711-4

448  Engineering the stereoisomeric structure of seed oil to mimic human milk fat.
https://www.pnas.org/content/116/42/20947

447  Data storage in DNA with fewer synthesis cycles using composite DNA letters.
https://www.nature.com/articles/s41587-019-0240-x

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