Biotechnology

Articles:

443  Engineered zinc finger nucleases achieve 98% knockout of a target gene in human T cells without detectable off-target activity.
https://www.nature.com/articles/s41587-019-0186-z

442  A divalent siRNA architecture enables sustained silencing of gene expression in deep regions of the brain.
https://www.nature.com/articles/s41587-019-0205-0

441  Encapsulation of Plasmid DNA by Nanoscale Metal–Organic Frameworks for Efficient Gene Transportation and Expression.
https://onlinelibrary.wiley.com/doi/10.1002/adma.201901570

440  RNA-guided DNA insertion with CRISPR-associated transposases.
https://science.sciencemag.org/content/365/6448/48

439  Programmable bacteria induce durable tumor regression and systemic antitumor immunity.
https://www.nature.com/articles/s41591-019-0498-z

438  Direct delivery of adenoviral CRISPR/Cas9 vector into the blastoderm for generation of targeted gene knockout in quail.
https://www.pnas.org/content/116/27/13288

437  An automated Raman-based platform for the sorting of live cells by functional properties.
https://www.nature.com/articles/s41564-019-0394-9

436  Increasing the accuracy of nanopore DNA sequencing using a time-varying cross membrane voltage.
https://www.nature.com/articles/s41587-019-0096-0

435  Increasing the specificity of CRISPR systems with engineered RNA secondary structures.
https://www.nature.com/articles/s41587-019-0095-1

434  An enzymatic pathway in the human gut microbiome that converts A to universal O type blood.
https://www.nature.com/articles/s41564-019-0469-7

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